Isolation, Identification and Sequence Analysis of Avian Reticuloendotheliosis Viruses in Taiwan
Date Issued
2005
Date
2005
Author(s)
Cheng, Wan-Hsin
DOI
zh-TW
Abstract
Avian reticuloendotheliosis virus (REV) infection is common, but not ubiquitous. The REV-infected chickens showed stunted, pale, and abnormal feather development. The principal lesions include runting, and atrophy of the thymus and bursa of Fabricius. No procedures have been applied in commercial practice for the control of RE, so the most important thing in the field is to distinguish the infected and uninfected chickens. In this study, we used commercial ELISA kits to detect REV-specific antibodies in color-breed chickens, breeder chickens, and broilers; meanwhile we detected the viral DNA in blood by polymerase chain reaction (PCR). Clinical materials, including whole blood, plasma, tumor tissues, and litter, were collected for virus isolation. We collected fowl pox virus (FPV) vaccines and Marek’s disease (MDV) vaccines provided by branch institute of animal drugs inspection animal health research institute for detection of REV contamination, and tried to isolate REV from these vaccines. The result of prevalence investigation showed 83% antibody positive rate in breeder chickens and 8% in color-breed chickens. The result of detection of REV in native color hybrid blood samples by PCR showed 29% positive rate, which meant that the infection of REV was popular in Taiwan. Two REVs, named 3122/03 and 3295/04, were isolated from clinical materials. Among eighteen FPV vaccines we collected, eleven of them showed REV LTR sequences integration and one REV was isolated from these vaccines. This isolate had cytopathic effect in DF1 cell line. No REV was isolated from seventeen MDV vaccines. We cloned partial envelope gene for the expression of recombinant protein used for the production of monoclonal antibody to develop antigen-capture ELISA. The results showed that we had selected three monoclonal antibodies which could recognize the recombinant protein and REVs. These monoclonal antibodies have potential for developing of antigen-capture ELISA. Based on the results of serology, REV DNA detection, and virus isolation, the REV infection in Taiwan is common. Specially, the isolation of REV from FPV vaccines shows a potential danger in the field.
Subjects
家禽網狀內皮症病毒
封套基因
avian reticuloendotheliosis virus
envelope gene
SDGs
Type
thesis
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