Polysaccharides purified from the submerged culture of Ganoderma formosanum stimulate macrophage activation and protect mice against Listeria monocytogenes infection
Resource
Biotechnol Lett.,33(11),2271-2278.
Journal
Biotechnology Letters
Journal Volume
33
Journal Issue
11
Pages
2271-2278
Date Issued
2011
Author(s)
Wang, Cheng-Li
Wang, Cheng-Li
Wang, CL
Pi, CC
Pi, Chia-Chen
Kuo, Chu-Wei
Kuo, CW
Zhuang, YJ
Zhuang, Yu-Jing
Khoo, Kay-Hooi
Khoo, KH
Liu, WH
Liu, Wen-Hsiung
Chen, CJ.
Abstract
The bioactive components of Ganoderma formosanum have not yet been characterized. We investigated the immunomodulatory activities of the extracellular polysaccharides produced from a submerged mycelial culture of G. formosanum. The polysaccharides were mainly composed of D-mannose, D-galactose and D-glucose. After gel filtration chromatography, three polysaccharide fractions (PS-F1, PS-F2 and PS-F3) were purified. PS-F2 stimulated mouse RAW264.7 macrophages to produce TNF-α and nitric oxide, and enhanced the phagocytic activity of macrophages. PS-F2 challenge in mice triggered an acute inflammatory response characterized by the recruitment of neutrophils and monocytes, which protected mice from subsequent infection of Listeria monocytogenes. The results indicate that the heteropolysaccharides produced by G. formosanum can activate the innate immune response on macrophages.
Type
journal article