The correlations between the expressions of c-kit and other emerging proteins in canine MCTs
Date Issued
2011
Date
2011
Author(s)
Hung, Chiao-Ling
Abstract
Mast cell tumors (MCTs) are one of the most common skin tumors in dogs. Although histological grading was an independent indicator for prognostic prediction, subjectivity in interpretation was considered as a major problem for a relatively low consistency in grading assignment. Moreover, great variation of biological behavior exhibited from grade II MCTs has been occupying a major component of the cases, impeding accurate prognosis prediction and has been regarded as a dilemma for deciding proper treatment strategies. Meanwhile, although KIT overexpression and c-kit mutation have been proven to be related to carcinogenesis of canine MCTs, the etiology remained unknown for most of the cases. Therefore, the necessity of exploring other potential parameters should be considered for advancing current information we’ve got. In this study, we evaluated the new proposed 2-tier grading systems in comparison with Patnaik system and modified Patnaik system. In addition, the emerging proteins including DOG1, KMO, Ki-67 and ENO1 were firstly investigated in the canine species for comparison with histological grading, KIT expression and clinical outcomes.
Current results showed that the use of 2-tier scheme was of benefit for discriminating aggressive tumors, but underestimation could occur in a subset of the tumors. Immunohistochemistry demonstrated the tendency of decreased expression of DOG1 and KMO in poorly-differentiated tumors in association with an overall decreased survival time. Tumors with negative DOG1 expression tended to have a shorter median disease-free interval less than 10 days whereas tumors with strong ENO1 expressions tended to have a shorter median survival time than the others. This observation provided a preliminary data of regulation of the emerging proteins. Further investigation is needed for validation of the preliminary result.
Subjects
MCTs
Histological grading
IHC
Type
thesis
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