Cytotoxicity of dehydrated ostrich acellular corneal stroma as a carrier material
Journal
Chinese Journal of Tissue Engineering Research
Journal Volume
17
Journal Issue
33
Pages
5995-6000
Date Issued
2013
Author(s)
Abstract
Background: Previous studies from Shaanxi Institute of Ophthalmology have shown that ostrich cornea has the advantages to be developed into the alternatives of human corneal material. Objective: To determine the potential toxic effects of ostrich corneal stromal scaffold on cells. Methods: Cell culture methods were used to culture L-929 cells in the extracts of ostrich acellular corneal stroma which was dried and dehydrated. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay was used to evaluate the growth and proliferation of cells after cultured for 1, 2 and 3 days. Results and Conclusion: After the cells were cultured in the extracts of ostrich acellular corneal stroma subjected to dryness and dehydration for 1, 3 and 5 days, and the toxicity level of cultured cells was graded as level 1. The cytotoxicity test was conducted according to the "National Standard of the People's Republic of China GB/T16886.5-2003". After cultured in the extracts of ostrich acellular corneal stroma, a small number of cells were round in shape and loosely adherent without intracytoplasmic granules, and cell lysis could be observed occasionally. The results of 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay showed that the ostrich acellular corneal stromal scaffold which was dried and dehydrated had level 1 of cytotoxicity and could be considered as a qualified material.
Type
journal article