Discrimination of wild and hatchery-reared black porgy (Acanthopagrus schlegeli) based on manganese of scales by graphite furnace atomic absorption spectrometer
Date Issued
2009
Date
2009
Author(s)
Chang, Cheng-Jung
Abstract
The restocking programs were conducted for many years in Taiwan. There are more than ten species released to the surrounding waters of Taiwan every year, i.e., black sea bream (Acanthopagrus schlegeli), Japanese sea bream (Pagrus major) and giant sea perch (Lates japonicas) etc. However, in the past, the effectiveness of the restocking program was not often evaluated except black sea bream and abalone. It is important to discriminate wild and hatchery-reared individual for the assessment of restocking program. In present, the major method to assess the effect of fry releasing was marked and recaptured. But this method could not identify the released individuals that were not marked before marked and released experiments. And there was another method using otolith elements analysis of magnesium (Mg) and manganese (Mn) to distinguish wild and hatchery-reared black porgy. However, this method was time consuming and too expensive. Therefore, it is important to find the discrimination methods requiring short operations time and inexpensive. This study examined the scale characteristics of five hatchery-reared and five recaptured marked-fish, and their manganese (Mn) of scales was investigated by graphite furnace atomic absorption spectroscope (GFAAS). According to the results, the discrimination criteria of captured black porgy were established. There were twenty captured individuals were analyzed in this study. Four unknown fish showed the stocking check, and ten showed that the Mn of scales was higher in the core area than in the periphery area among all fish. Thereby, this study suggested ten individuals may be hatchery-reared fish and ten individual may be wild fish. This study suggests when discriminate original unknown fish, first, observed fish scale then analyzed Mn in scale. Finally, when there are still some doubt samples, Mg and Mn analysis will be necessary. By this way, time and expenditure can be reduced and the contribution of the previously unmark releasing fish be estimated. It will do merits to future resource administration and releasing projects.
Subjects
black porgy, Acanthopagrus schlegeli
scales
marked and release
GFAAS
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