A manual and portable centrifuge combined with a paper-based immunoassay for myocardial infarction diagnosis
Journal
Chemical Engineering Journal
Journal Volume
409
Date Issued
2021
Author(s)
Abstract
Myocardial infarction (MI) causes hundreds of thousands of deaths annually. Early diagnosis of MI by detecting the concentration of troponin I in plasma can help limit mortality rates. However, plasma separation and early diagnosis are particularly difficult to achieve in resource-limited settings. In this study, we demonstrate a manual and portable centrifuge along with a paper analytical device to measure troponin I for early MI diagnosis. With this so-called “Handfuge,” pure plasma can be achieved with a yield of up to 47.1% and purity as high as 99.9% in less than 3 min without electricity. The resulting plasma can then be directly used for detecting troponin I using a paper-based enzyme-linked immunosorbent assay (ELISA), with a detection limit as low as 0.03 ng/mL. The whole detection process consumes just 10 ?L of blood, which is similar to the volume obtained by a clinical finger-prick. The proposed centrifuge together with the paper analytical device possesses the potential to replace existing techniques in troponin I detection for early MI diagnoses in resource-limited regions of the world. ? 2020 Elsevier B.V.
Subjects
Analytic equipment; Cardiology; Diagnosis; Detection limits; Detection process; Early diagnosis; Enzyme linked immunosorbent assay; Mi diagnosis; Mortality rate; Myocardial Infarction; Plasma separation; Centrifuges
SDGs
Type
journal article