Effects of Temple Particles on Inflammatory Reaction and Endothelial Dysfunction by Human Coronary Artery Endothelial Cell
Date Issued
2005
Date
2005
Author(s)
Chuang, Hsiao-Chi
DOI
zh-TW
Abstract
Objective：To investigate size distribution and concentrations of particulate and particle-phase polycyclic aromatic hydrocarbons(PAHs) measured in the temple(temple particles) and to determine the inflammatory reaction and endothelial dysfunction related to them.
Methods：We used Micro-Orifice Uniform Deposit Impactors(MOUDI) to collect particles from a major temple in Taipei, Taiwan. The concentrations of particulte-phase PAHs were dtermined by using a gas chromatography/mass spectrometry(GC/MS) method. Human coronary artery endothelial cells(HCAEC) were exposured to particle extracts at 25 and 50 µg/mL for 4 hr, and interleukin-6(IL-6), nitrite oxide(NO), and endothelin-1(ET-1) concentrations in the basal medium were measured by the enzyme-link immunosorbent assay(ELISA) method.
Results：Mean concentrations of PM10-1.0, PM1.0-0.1 and PM0.1were 28.9 ± 6.7, 121.0 ± 37.3 and 5.2±3.2 µg/m3, respectively. The ratio of PM1.0/PM10 were 81.2 ± 5.3％. Mean concentrations among total PAHs in PM10-1.0, PM1.0-0.1 and PM0.1were 0.080 ± 0.002, 0.224 ± 0.003 and 0.011 ± 0.001 µg/m3, respectively. Percentage among five-ring PAHs in PM10-1.0, PM1.0-0.1 and PM0.1 were 55.4, 40.7 and 45.7％, respectively. The benzo[b]fluoranthene concentrations were the highest among all 16 PAHs in PM10, PM1.0 and PM0.1. 50 µg/mL PM induced more IL-6, NO and ET-1 release than 20 ug/mL PM. Among thre PM fractions, PM1.0-0.1 stimulation resulted in higher IL-6 and ET-1 production than PM10-1.0 and PM0.1. By contrast, PM0.1 stimulation resulted in lower NO production than PM10-1.0 and PM1.0-0.1. Total PAHs were negatively associated with NO(R2=0.58) and ET-1(R2=0.68) production, respectively. For PAHs in PM1.0, anthracene(R2=0.48), pyrene(R2=0.47), benzo[b]fluoranthene(R2=0.51) and benzo[a]pyrene(R2=0.42) were negatively associated with NO production. By contrast, a naphthalene(R2=0.89), acenaphthylene(R2=0.79), acenaphthene(R2=0.82), fluorene(R2=0.75), anthracene (R2=0.97) and dibenz[a,h]anthracene(R2=0.71) were negatively associated with ET-1 release.
Conclusions：Our study found that major size of temple particles was PM1.0. Particulate PAHs in the temple was mainly composed of five-ring PAHs, and benzo[b]fluoranthene was the major component of five-ring PAHs. Moreover, size and PAHs of temple particles were both important factors related to inflammatory reaction and endothelial dysfunction by HCAEC.
Subjects
寺廟
懸浮微粒
多環芳香烴化合物
冠狀動脈內皮細胞
細胞激素IL-6
一氧化氮
內皮素-1
Temple
Particulate matter(PM)
Polycyclic aromatic hydrocarbons(PAHs)
Human coronary artery endothelial cell(HCAEC)
Interleukin-6(IL-6)
Nitrite oxide(NO)
Endothelin-1(ET-1)
Type
thesis
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