Construction of an Economic Scanning Electrochemical Microprobe System and the Application on Dielectrophoresis of Red Blood Cells
Date Issued
2007
Date
2007
Author(s)
Shih, Syuan-He
DOI
zh-TW
Abstract
An economic scanning probe system has been constructed for manipulating biomolecules and/or cells under microscopic observation; the system included a home-made microelectrodes, PC-controlled 3D-scanning system, the user interface and a dielectrophoresis chamber for direct observation.
The micro-electrode was made by electrochemical etching (3Vrms for 9mins in 1M NaOH) of a pencil lead (0.5mm in diameter), and then the sharpened pencil lead was sealed (insulated) within a glass capillary using cyanoacrylate adhesive. The electrode tip was polished with diamond lapping films to reveal its micro-scaled electroactive region (~10μm in diameter). Its electrochemical characteristics are similar to a commercial carbon paste micro-disk electrode.
The 3D-scanning system was driven with three stepped motors controlled by a user interface program written under Labview
Subjects
微鉛筆芯電極
鉛筆芯蝕刻
紅血球細胞介電泳操控
經濟型細胞分子操控平台
Microelectrodes
Dielectrophoresis
Cell manipulation
Indium tin oxide
Type
thesis
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