Gonadotropin-releasing hormone (GnRH) promoter analysis of Anguilla japonica
Date Issued
2010
Date
2010
Author(s)
Liu, Ping-Fu
Abstract
Anguilla japonica is one of important species in Asia aquaculture. However, glass eels production is continuously declining, resulting in great impact on the development of eel aquaculture industry. In order to reach a sustainable eel production, we should develop complete artificial propagation. For this reason we need to know the mechanisms of eel nature maturation. Like other teleost, the center of eel reproduction endocrine control system is hypothalamus-pituitary-gonad (HPG) axis, and GnRH is the major regulator in HPG axis. For this reason, this study focuses on promoter analysis of eel GnRH gene. In the promoter assay, the upstream sequences before GnRH start coding will be available from 5’-Genome Walking. Difference of GnRH promoter area is zonated by specific primers and PCR. After that, PCR product is constructed into pGL3-basic vector then transfect in GT1-7 cell line. Promoter region of GnRH is determinated by assaying the firefly luciferase activity. The results indicate that the critical region for GnRH transcription locates in -388 to -499 that contain the important transcription factors Oct-1 and Pbx-1a for GnRH expression. Moreover, sex-steroids maybe indirectly activate GnRH promoter via this region from -388 to -499.
Subjects
Gonadotropin-releasing hormone
Promoter
Transcription factors
Stimulation of sex-steroids
File(s)![Thumbnail Image]()
Loading...
Name
ntu-99-R97b45016-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):ff96c0f46856c901bdf7f218b7eb71f8