Cloning and Characterization of Carp Intersex Gene
Date Issued
2006
Date
2006
Author(s)
Wei, Liang-Tai
DOI
zh-TW
Abstract
Abstract
The sex-determination of Drosophila melanogaster has been known clearly in previous studies. There is a gene called intersex (ix), which functions together with doublesex (dsx) at the bottom of the hierarchy in the sex deternination females. Drosophila ix is expressed in both sex, but only functions in female somatic cell. It has no effect on male. There is no recognizable DNA-binding domain on the amino acid sequence of ix for binding to DNA, but it contains a region like transcriptional activation domains to interact with other proteins, and ix might play a similar role as the C-terminal of DSXM when acts together with DSXF.
Mediator complex was found in yeast, it is a protein complex composed over 20 subunits, and plays an essential role in the regulation of transcription. Brower et al. (2002) and Wang et al. (2004) also have cloned a navel gene of mammalian Mediator complex subunit, which is the homolog of Drosophila ix. Northern blot analysis demonstrated that ix is expressed in various tissues, and the protein is localized mainly to nucleus, supporting its function as a transcriptional regulator.
Our laboratory also found a gene like Drosophila intersex in the testis of Cyprinus carpio (cIX). According to the significant function played by Drosophila ix in sex-determination, we are interested in the function of this gene in carp. To study the functions of cIX further, I cloned the full-length cDNA of cIX. The recombinant protein is produced and purified to serve as the antigen to induce antibody. The results of PCR and western blot analysis demonstrated that cIX is broadly existing in various tissue of carp, when the tissue specificity is not distinct. I also found that cIX has two mainly form, and the alignment of the homologs of other species indicates that IX proteins are conserved in C-terminal, and shows variation in N-terminal. The meaning of these information needs further study.
Subjects
鯉魚
intersex
carp
mediator complex
Type
other
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