Growth inhibition of human endothelial cells by the phyto-oestrogen biochanin A, a metabolite of genistein
Journal
British Journal of Nutrition
Journal Volume
85
Journal Issue
5
Pages
615-620
Date Issued
2001
Author(s)
Abstract
This study examined the growth regulatory effects of a phyto-oestrogen, biochanin A, on a transformed human endothelial cell line ECV304 in vitro. Biochanin A was found to inhibit cell proliferation in a dose-dependent fashion and this effect was influenced by the concentration of serum present in the culture medium. In the absence of serum, the calculated IC50 of biochanin A was 0.18 ± 0.1 μM compared to an IC50 of 35 ± 5 μM at 10 % serum. At low cell density, the growth inhibitory effects of biochanin A were more evident than at high cell density. Co-administration of a synthetic oestrogen diethylstilboestrol with biochanin A did not suppress the growth regulatory effects of biochanin A treatment. We conclude that biochanin A inhibits the cell proliferation of human endothelial cells at concentrations that are physiologically achievable in vivo and that this effect may play an important role in the cancer-preventing activity of the phyto-oestrogens.
Subjects
Cell proliferation; Endothelial cells; Phyto-oestrogen
SDGs
Other Subjects
biochanin A; diethylstilbestrol derivative; drug metabolite; genistein; phytoestrogen; article; cancer prevention; cell density; cell growth; cell proliferation; concentration response; culture medium; drug activity; drug antagonism; endothelium cell; growth inhibition; human; human cell; IC 50; in vitro study; in vivo study; inhibition kinetics; regulatory mechanism
Type
journal article