行政院國家科學委員會專題研究計畫期中進度報告:眼睛翳狀贅肉結膜下彈性素堆積之基因轉殖鼠模式之建立(2/3)
Date Issued
2003
Date
2003
Author(s)
王一中
DOI
912314B002190
Abstract
Pterygium is a worldwide ocular surface disease, an ocular disorder
that occurs more often in the population of tropical and subtropical region
1. It often causes irritating or inflamed ocular symptoms, and also
accompanies with local drying of corneas and conjunctivas.
Elastin is a major component of connective tissue in large arteries, lung,
skin, and ligmenetum, and provides elasticity in these tissues 2. In normal
skin, elastic fibers are found in the dermal connective tissue in only small
amounts. In photodamaged skin, on the other hand, elastic fibers are the
most prominent component of extracellular matrix and are located in the
superficial to mid-dermis as an amorphous blue staining area on routine
hematoxylin-eosin staining 3,4
Accumulation of elastin in solar elastosis of photodamaged skin has
also been demonstrated in clinical situation and experimental animals, by
both immunohistochemistry and molecular biology techniques5,6. It is
likely that the pathologic changes in conjunctiva in response to chronic
UV irradiation are similar to those in chronically sun-damaged skin. For
example, elastodysplasia and elastodystrophy are known to be present in
the subepithelial connective tissue of pinguecular part in pterygium, and
similar pathologic elastin accumulation is found in chronically
sun-damaged skin 7. Therefore,we speculate that the pathogenesis of
pterygia may be the same as photodamaged skin.
The increase in elastin mRNA levels has been demonstrated in
fibroblasts of photoaged skin, as compared with those from skin of
normal subjects. This increased level of elastin mRNA in sun-damaged
skin is resulted from enhanced elastin promoter activity. Although we
found that post-transcriptional regulation of elastin promoter plays an
important role in the formation of elastoid degeneration of pterygium, we
still want to investigate the possibility of transcriptional regulation of
elastin promoter. We will try to use transgenic model to study the elastin
expression in pterygium.8
In the present study, we tried to generate a transgenic mice carry a 2.8
kb human elastin promoter with a GFP reporter gene, and apply this mice
to the model of pterygial formation. We also try to generate an ablation
model of conjunctival fibroblasts by using DTA constructs.
SDGs
Publisher
臺北市：國立臺灣大學醫學院眼科
Type
journal article
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