花粉,種子與植物體耐旱機制之研究(2/3)
Date Issued
2004-07-31
Date
2004-07-31
Author(s)
DOI
922313B002041
Abstract
Selaginella tamariscina, a resurrection plant, can survive severe water stress. To
investigate the mechanism of desiccation tolerance of resurrection plant, differential
display was employed to find the dehydration induced genes in S. tamariscina during
dehydration. About 100 dehydration induced cDNA clones were collected. Among
them, the full length of 12 strongly induced cDNA clones were obtained by using
5’-race technology. These12 full-length cDNA clones were identified because high
similarity to the orthologs in other plants. Northern blot analysis was also applied to
conform that these cDNA clones were actually dehydration activated. In the future,
chromosome walking will be executed to obtain the promoter sequence of these
dehydration inducible genes, and the cis-acting element will be analyzed to elucidate
the molecular mechanism of desiccation tolerance of S. tamariscina.
Publisher
臺北市:國立臺灣大學植物科學研究所
Type
journal article
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