Electric field-stimulated Raman scattering enhancing biochips fabricated by Au nano-islands deposited on laser-scribed 3D graphene for uremic toxins detection
Journal
Journal of the Taiwan Institute of Chemical Engineers
Date Issued
2023-01-01
Author(s)
Juang, Ruey Shin
Wang, Kuan Syun
Kuan, Tsai Yu
Chu, Yu Ju
Hardiansyah, Andri
Liu, Shou Hsuan
Liu, Ting Yu
Abstract
Background: The detection of uremic toxins is important for early-stage diagnosis of chronic kidney disease. However, Raman spectroscopy is shortcoming of the sensitivity for detecting the trace analytes. The electric field-induced chemical surface-enhanced Raman scattering (SERS) enhancement (EC-SERS) detection is great way to solve and investigates uremic toxins. Methods: In this work, various thickness (5–25 nm) of Au nano-islands were deposited on the 3D laser-scribed graphene (LSG) substrate for increasing the sensitivity and reproducibility of SERS detection. Particularly, applying the electric-field stimulus in Au-LSG SERS substrate allows to further amplify the SERS signals, and measure the EC-SERS signals of biomolecules. Significant findings: The results show that 20 nm of Au nano-islands coated on LSG substrate obtains the highest SERS enhancement effects, and successfully detects the dye molecules (rhodamine 6G, R6G) and uremic toxins (urea and creatinine). The EC-SERS signals of R6G would enhance 17 times at the potential of −1.3 V, compared to SERS signals without applying an electric field. Moreover, the urea also displays 4 times higher at the potential of −0.2 V. The detecting molecules could be selected to enhance SERS signals by different voltages, showing the capability of selectively detecting biomolecules, which can solve the problem of complex sample pretreatment.
Subjects
Electric field-induced chemical surface-enhanced Raman scattering enhancement (EC-SERS) detection | Gold nano-islands | Laser-scribed grapheme | Uremic toxins
SDGs
Type
journal article