Determinations of Microbial Volatile Organic Compounds by a Solid-Phase Microextraction Device
Date Issued
2007
Date
2007
Author(s)
Juan, Pei-Hua
DOI
en-US
Abstract
Indoor air pollution is a major problem of public health nowadays because people usually spend more than 80% of time indoor. Fungus is an important indoor air pollutant and is known to be a fundamental allergen of asthma. Microbial volatile organic compounds (MVOCs) which are the metabolites of fungi can also be used to relate the presence of fungi even there is no spore. To improve the quality of indoor air, setting standards for indoor biological hazards are very important. Therefore the purpose of this study is to develope the sampling and analysis technique for indoor MVOCs based on solid-phase microextraction (SPME) device.
SPME technique was utilized as a rapid air sampling sampler while its performances on major indoor MOVCS, including 1-butanol, 1-octen-3-ol, 2-methyl-1-propanol, 2-heptanone, 2-pentylfuran, 2-hexanone and 3-methyl-1-butanol were validated. 65 μm PDMS/DVB fiber was selected. Known concentration of MVOCs were generated in dynamic vapor generation system for the validations of SPME rapid air sampling samplers. After sampling, the sampler was inserted into the injection port of gas chromatography with mass spectrometry (GC/MS) for thermal desorption and analysis.
The results showed that the desorption efficiency was 100% when the time for thermal desorption was 1.5 min. It was also found that different ranges of linearity were observed for all the MVOCs at different sampling conditions. Under the sampling time of 10 seconds to 60 minutes, the experimental sampling rates were 0.05~1.75 cm3/s, 0.41~1.04 cm3/s, 0.55~0.97 cm3/s and 0.27~1.07 cm3/s for 1-butanol, 2-heptanone, 2-pentylfuran and 2-hexanone, respectively. Under the sampling time of 20 seconds to 40 minutes, the experimental sampling rates were 0.50~0.98 cm3/s and 0.26~2.19 cm3/s for 1-octen-3-ol and 3-methyl-1-butanol, respectively. The experimental sampling rates was 0.06~1.21 cm3/s for 2-methyl-1-propanol under the sampling time of 10 seconds to 40 minutes. Moreover, the instrumental detection limit (IDL) ranged between 0.20 and 1.56 ng/μL while the method detection limit (MDL) ranged between 1.15 and 3.74 ng/μL. The designed rapid air sampling device for MVOCs has the advantages of SPME technique which can be used for monitoring indoor air quality (IAQ) in the future.
Subjects
固相微萃取
外推式採樣
MVOCs
氣相層析質譜儀
solid-phase microextraction
SPME rapid air sampling
GC/MS
SDGs
Type
thesis
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