Supplementation of Chitosan Alleviates High-Fat Diet-Enhanced Lipogenesis in Rats via Adenosine Monophosphate (AMP)-Activated Protein Kinase Activation and Inhibition of Lipogenesis-Associated Genes
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
63
Journal Issue
11
Pages
2979-2988
Date Issued
2015
Author(s)
Abstract
This study investigated the role of chitosan in lipogenesis in high-fat diet-induced obese rats. The lipogenesis-associated genes and their upstream regulatory proteins were explored. Diet supplementation of chitosan efficiently decreased the increased weights in body, livers, and adipose tissues in high-fat diet-fed rats. Chitosan supplementation significantly raised the lipolysis rate; attenuated the adipocyte hypertrophy, triglyceride accumulation, and lipoprotein lipase activity in epididymal adipose tissues; and decreased hepatic enzyme activities of lipid biosynthesis. Chitosan supplementation significantly activated adenosine monophosphate (AMP)-activated protein kinase (AMPK) phosphorylation and attenuated high-fat diet-induced protein expressions of lipogenic transcription factors (PPAR-γ and SREBP1c) in livers and adipose tissues. Moreover, chitosan supplementation significantly inhibited the expressions of downstream lipogenic genes (FAS, HMGCR, FATP1, and FABP4) in livers and adipose tissues of high-fat diet-fed rats. These results demonstrate for the first time that chitosan supplementation alleviates high-fat diet-enhanced lipogenesis in rats via AMPK activation and lipogenesis-associated gene inhibition. ? 2015 American Chemical Society.
Subjects
AMP-activated protein kinase; chitosan; gene expression; high-fat diet; lipogenesis
SDGs
Other Subjects
Biochemistry; Chemical activation; Chitin; Chitosan; Enzyme activity; Enzymes; Genes; Histology; Nutrition; Proteins; Rats; Tissue; Transcription; Activated proteins; Adenosine monophosphate; Adipocyte hypertrophy; AMP-activated protein kinase; High-fat diet; Lipogenesis; Lipoprotein lipase; Regulatory protein; Gene expression; Rattus; chitosan; FABP4 protein, rat; fatty acid binding protein; hydroxymethylglutaryl coenzyme A reductase kinase; peroxisome proliferator activated receptor gamma; sterol regulatory element binding protein 1; adipose tissue; adverse effects; analysis; animal; diet supplementation; drug effects; enzyme activation; enzymology; gene expression; genetics; human; lipid diet; lipogenesis; liver; male; metabolism; obesity; pathophysiology; rat; Sprague Dawley rat; Adipose Tissue; AMP-Activated Protein Kinases; Animals; Chitosan; Diet, High-Fat; Dietary Supplements; Enzyme Activation; Fatty Acid-Binding Proteins; Gene Expression; Humans; Lipogenesis; Liver; Male; Obesity; PPAR gamma; Rats; Rats, Sprague-Dawley; Sterol Regulatory Element Binding Protein 1
Type
journal article