Ptenb Mediates Gastrulation Cell Movements via Cdc42/AKT1 in Zebrafish
Resource
PLOS ONE, 6(4), e18702
Journal
PLoS ONE
Journal Volume
6
Journal Issue
4
Date Issued
2011
Date
2011
Author(s)
Yeh, Chen-Min
Liu, Yi-Ching
Chang, Ching-Jen
Lai, Shih-Lei
Hsiao, Chung-Der
Cooney, Austin John
Abstract
Phosphatidylinositol 3-kinase (PI3 kinase) mediates gastrulation cell migration in zebrafish via its regulation of PIP2/PIP3 balance. Although PI3 kinase counter enzyme PTEN has also been reported to be essential for gastrulation, its role in zebrafish gastrulation has been controversial due to the lack of gastrulation defects in pten-null mutants. To clarify this issue, we knocked down a pten isoform, ptenb by using anti-sense morpholino oligos (MOs) in zebrafish embryos and found that ptenb MOs inhibit convergent extension by affecting cell motility and protrusion during gastrulation. The ptenb MO-induced convergence defect could be rescued by a PI3-kinase inhibitor, LY294002 and by overexpressing dominant negative Cdc42. Overexpression of human constitutively active akt1 showed similar convergent extension defects in zebrafish embryos. We also observed a clear enhancement of actin polymerization in ptenb morphants under cofocal microscopy and in actin polymerization assay. These results suggest that Ptenb by antagonizing PI3 kinase and its downstream Akt1 and Cdc42 to regulate actin polymerization that is critical for proper cell motility and migration control during gastrulation in zebrafish. © 2011 Yeh et al.
SDGs
Other Subjects
antisense oligonucleotide; phosphatidylinositol 3 kinase; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; protein AKT1; protein Cdc42; protein kinase B; unclassified drug; actin; antisense oligonucleotide; phosphatidylinositol 3 kinase; phosphoprotein phosphatase; protein Cdc42; protein kinase B; Ptenb protein, zebrafish; zebrafish protein; actin polymerization; animal cell; animal tissue; article; cell motion; controlled study; embryo; enzyme activity; enzyme inhibition; gastrulation; gene; gene overexpression; gene silencing; nonhuman; protein function; ptenb gene; zebra fish; animal; animal embryo; biological model; cell motion; cytology; dominant gene; drug antagonism; drug effect; embryo development; enzyme activation; enzymology; genetics; human; metabolism; polymerization; prenatal development; protein synthesis; signal transduction; zebra fish; Danio rerio; Actins; Animals; cdc42 GTP-Binding Protein; Cell Movement; Embryo, Nonmammalian; Embryonic Development; Enzyme Activation; Gastrulation; Gene Knockdown Techniques; Genes, Dominant; Humans; Models, Biological; Oligonucleotides, Antisense; Phosphatidylinositol 3-Kinases; Phosphoprotein Phosphatases; Polymerization; Protein Biosynthesis; Proto-Oncogene Proteins c-akt; Signal Transduction; Zebrafish; Zebrafish Proteins
Type
journal article
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