Liver epithelial cells inhibit proliferation and invasiveness of hepatoma cells
Journal
Oncology Reports
Journal Volume
35
Journal Issue
3
Pages
1622-1628
Date Issued
2016
Author(s)
Abstract
Hepatocellular carcinoma (HCC) is a worldwide malignancy with poor prognosis. Liver progenitors or stem cells could be a potential therapy for HCC treatment since they migrate toward tumors. Rat liver epithelial (RLE) cells have both progenitor and stem cell-like properties. Therefore, our study elucidated the therapeutic effect of RLE cells in rat hepatoma cells. RLE cells were isolated from 10-day old rats and characterized for stem cell marker expression. RLE cells and rat hepatoma cells (H4-IIE-C3 cells) were co-cultured and divided into four groups with different ratios of RLE and hepatoma cells. Group A had only rat hepatoma cells as a control group. The ratios of rat hepatoma and RLE cells in group B, C and D were 5:1, 1:1 and 1:5, respectively. Effective inhibition of cell proliferation and migration was found in group D when compared to group A. There was a significant decrease in Bcl2 expression and increase in late apoptosis of rat hepatoma cells when adding more RLE cells. RLE cells reduced cell proliferation and migration of rat hepatoma cells. These results suggested that RLE cells could be used as a potential cell therapy.
Subjects
Apoptosis; Bcl2; Cell therapy; Hepatocellular carcinoma; Rat liver epithelial cells
SDGs
Other Subjects
protein bcl 2; protein bcl 2; animal cell; apoptosis; Article; cancer growth; cell interaction; cell invasion; cell migration; cell proliferation; cell structure; controlled study; epithelium cell; hepatoma cell; liver epithelial cell; mesenchymal stem cell; nonhuman; priority journal; protein expression; rat; tumor promotion; animal; biological therapy; biosynthesis; cancer stem cell; Carcinoma, Hepatocellular; cell proliferation; coculture; epithelium cell; gene expression regulation; genetics; human; liver cell; Liver Neoplasms; metabolism; pathology; tumor cell line; Animals; Apoptosis; Carcinoma, Hepatocellular; Cell Line, Tumor; Cell Proliferation; Cell- and Tissue-Based Therapy; Coculture Techniques; Epithelial Cells; Gene Expression Regulation, Neoplastic; Hepatocytes; Humans; Liver Neoplasms; Neoplastic Stem Cells; Proto-Oncogene Proteins c-bcl-2; Rats
Type
journal article