Profiles of PGH-α, GTH I-β, and GTH II-β mRNA transcript levels at different ovarian stages in the wild female Japanese eel Anguilla japonica
Resource
General and Comparative Endocrinology, 133(1), 8-16
Journal
General and Comparative Endocrinology
Journal Volume
133
Journal Issue
1
Pages
8-16
Date Issued
2003
Date
2003
Author(s)
Abstract
The complete complementary DNA (cDNA) encoding pituitary gonadotropin II-β subunit (GTH II-β) of Japanese eel Anguilla japonica was cloned and sequenced, and the profiles of pituitary glycoprotein hormone α subunit (PGH-α), GTH I-β, and GTH II-β mRNA transcript levels at different stages of ovarian development before vitellogenesis in the wild females were investigated. The maturity of female eels was divided into four stages: juvenile, sub-adult, pre-silver, and silver stages based on ovarian development and skin color. The GTH II-β cDNA was cloned by reverse transcription and polymerase chain reaction (RT-PCR) amplification from total pituitary RNA. The full length GTH II-β cDNA was obtained using 5′- and 3′-rapid amplification of cDNA ends. The cloned eel GTH II-β cDNA consists of 646bp nucleotides, including 53bp nucleotides of 5′-untranslated region (UTR), 423bp of open reading frame, and 170bp nucleotides of 3′-UTR followed by a poly(A) tail. It encodes a 140-amino acid precursor molecule of GTH II-β subunit with a putative signal peptide of 24 amino acids and a mature peptide of 116 amino acids. RT-PCR analysis showed that the pituitary transcript levels of α subunit steadily increased during eel silvering. The expression of GTH I-β and II-β mRNA levels, however, varied in different ovarian developmental stages. The mRNA expression of both GTH I-β and GTH II-β were detectable in juvenile stage. The expression levels of GTH II-β mRNA, but not GTH I-β, were significantly increased in sub-adult stage. The transcript levels of GTH I-β and II-β subunits further increased in pre-silver and silver stages. We demonstrated for the first time that the differential transcription patterns of pituitary PGH-α, GTH I-β, and GTH II-β mRNAs occur during silvering of the wild female Japanese eels. © 2003 Elsevier Science (USA). All rights reserved.
Subjects
Japanese eel (Anguilla japonica)
silvering
ovarian development
GTH I-β subunit
GTH II-β subunit
α subunit
gene expression
SDGs
Other Subjects
amino acid; complementary DNA; gonadotrophin 1 beta; gonadotrophin 2 beta; gonadotropin; hypophysis hormone; messenger RNA; nucleotide; pituitary glycoprotein hormone alpha; RNA; signal peptide; unclassified drug; 3' untranslated region; 5' untranslated region; alpha chain; anguilla japonica; animal cell; animal tissue; article; beta chain; developmental stage; eel; female; gene amplification; gene expression; genetic code; genetic transcription; juvenile; maturation; molecular cloning; molecule; nonhuman; nucleotide sequence; open reading frame; ovary development; precursor; priority journal; reverse transcription polymerase chain reaction; skin color; vitellogenesis
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
1004.pdf
Size
310.42 KB
Format
Adobe PDF
Checksum
(MD5):d090c3771abf5f596988e267d9bac4fe