Effects of dietary flavonoids, luteolin and quercetin on the reversal of epithelial-mesenchymal transition in A431 epidermal cancer cells
Date Issued
2011
Date
2011
Author(s)
Lin, Yung-Sheng
Abstract
Highly invasive A431-III cells, which are derived from parental A431-P cells, were originally isolated via three successive passages through a Boyden chamber using a Matrigel-coated membrane support. The greater invasion potential exhibited by A431-III cells was due to their increased ability to spread/migrate, which was associated with enhanced MMP activity. The tumor progression events evoked by A431-P cells compared to A431-III cells may help identify useful strategies for evaluating the EMT and these cell lines could be a reliable model for evaluating tumor metastasis events. Employing this approach, we evaluated the effects of luteolin and quercetin using the A431-P/A431-III EMT model. These flavonoids reversed cadherin switching, downregulated EMT markers and nullified the invasion ability of A431-III cells. Overexpression of MMP-9 resulted in induction of the EMT in A431-P cells and this could be reversed by treating with luteolin or quercetin. Co-treatment of A431-P and A431-III cells with EGF plus luteolin or quercetin resulted in a more epithelial-like morphology, led to reduced levels of the EGF-induced markers of the EMT and caused the restoration of cell-cell junctions. E-cadherin was decreased by EGF, but increased by luteolin and quercetin. Our results suggest that luteolin and quercetin are potentially beneficial agents that target and prevent the occurrence of the EMT in epidermal carcinoma cells. These chemicals also have the ability to attenuate tumor progression in A431-III cells. In conclusion, luteolin and quercetin show inherent potential as chemopreventive/anti-neoplastic agents and do this by abating tumor progression through a reversal of EMT.
Subjects
Flavonoids
Luteolin
Quercetin
Antioxidant
EMT
Invasion
Cadherin
Transcription
MMP
SDGs
Type
thesis
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