Controlled release of human adipose-derived stem-cells from thermosensitive chitosan hydrogel
Date Issued
2014
Date
2014
Author(s)
Lin, Wei-Jhih
Abstract
In the research of regenerative medicine, it is promising treatment to apply thermosensitive hydrogel encapsulated with stem cells to improve the cell viability. However, the application might limit the effect of cell-cell interaction. The present study focuses on whether the adipose stem cells (ASC) release would benefit wound healing. Therefore, the thermosensitive chitosan hydrogel was blended with hydrophilic material such as gelatin and chitosonic acid (CA) in order to form porous structure. The research aims to construct the stable ASC release systems and to discuss the effect of wound healing and angiogenesis ability. To maintain thermosensitive character of chitosan hydrogel, the study blended chitosan with 4% gelatin and 1% chitosonic acid, and the hydrophilic materials resulted in porous structure under the scanning electron microscope (SEM) image. We found that the blending concentration of gelatin was proportional to the pores of chitosan hydrogel. According to the number of released cells from the hydrogel, the gelatin blending with porous structure was not only beneficial to the effect of ASC release profile, but also enhanced viability and proliferation of ASC. As for the function of wound healing to ASC release systems, the present study demonstrated that the release of ASC could facilitate wound closure by co-culturing fibroblast (Hs68) with ASC encapsulated in chitosan-gelatin hydrogel. In terms of angiogenesis, the in vitro study of co-culture endothelial cell (SVEC4-10) with ASC release systems observed SVEC4-10 differentiated into network tube formation, indicating it had potential in angiogenesis. For in vivo study, the angiogenesis ability was confirmed with chick embryo chorioallantoic membrane (CAM) assay. The quantification of the average capillary density showed that ASC released from chitosan hydrogel promoted angiogenesis and wound healing in tissue engineering.
Subjects
adipose stem cell
thermosensitive hydrogel
chitosan
cell release
angiogenesis
SDGs
Type
thesis
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