行政院國家科學委員會專題研究計畫期中進度報告:雞之視覺剝奪性近視的基因表現及蛋白體研究(2/3)
Date Issued
2004
Date
2004
Author(s)
楊長豪
DOI
922314B002162
Abstract
Purpose: Myopia is characterized by excessive elongation of eyeball. Increased DNA
synthesis and cellular proliferation had been shown by previous studies; however, the
underlying mechanism was still unknown. In this experiment, subtraction
hybridization technique was used to evaluated the differential gene expression
between myopic and control eyes.
Methods: Form deprivation myopia was induced in neonatal chicks by occlusion
their right eyes with goggle. The chicks were sacrificed 2 weeks later and the eyes
were enucleated. Sclera was harvested and mRNA was extracted. The differential
gene expression between myopic eyes and control eyes was examined by subtraction
hybridization methods.
Results: With subtraction hybridization technique, 5 out of 400 differential
expressed clones were picked up. The clones were sequenced and compared with
Gene bank data. The candidate genes included angiopoietin gene, caspase 6 gene,
prkdc gene for DNA-dependent proteinase kinase, myosin heavy chain gene, and
alpha1 integrin gene.
Conclusions: Subtraction hybridization is a powerful tool to study gene expression
in myopia. The significant of these genes in myopia development need further
investigation.
Publisher
臺北市：國立臺灣大學醫學院眼科
Type
journal article
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