Microbiological transformation of diosgenin by resting cells of filamentous fungus, Cunninghamella echinulata CGMCC 3.2716
Journal
Journal of Molecular Catalysis B: Enzymatic
Journal Volume
67
Journal Issue
3月4日
Pages
251-256
Date Issued
2010
Author(s)
Abstract
Microbial transformation of the steroidal sapogenin diosgenin (1) by resting cells of the filamentous fungus, Cunninghamella echinulata CGMCC 3.2716 was studied. Four metabolites were isolated and unambiguously characterized as (25R)-spirost-5-ene-3β,7β-diol-11-one (2), (25R)-spirost-5-ene- 3β,7β-diol (3), (25R)-spirost-5-ene-3β,7β,11α-triol (4), and (25R)-spirost-5-ene-3β,7β,12β-triol (5), by various spectroscopic methods (1H, 13C NMR, DEPT, 1H-1H COSY, HMBC, HSQC and NOESY). Compound 2 is a new metabolite. The NMR data and full assignment for the known metabolites (25R)-spirost-5-ene-3β,7β-diol (3) and (25R)-spirost-5-ene-3β, 7β,11α-triol (4) are described here for the first time. The biotransformation characteristics observed included were C-7β, C-11α and C-12β hydroxylations. Compounds 1-5 exhibited no significant cytotoxic activity to human glioma cell line U87. © 2010 Elsevier B.V. All rights reserved.
SDGs
Type
journal article