Efficient spin-spin scalar coupling mediated C-13 homonuclear polarization transfer in biological solids without proton decoupling
Journal
Solid State Nuclear Magnetic Resonance
Journal Volume
29
Journal Issue
4
Pages
278-282
Date Issued
2006
Author(s)
Abstract
We demonstrate that an efficient C' C alpha polarization transfer based on J-coupling can be realized under fast magic-angle spinning (MAS) condition without 1H decoupling. Experimental results are presented for model crystalline compounds as well as a non-crystalline 17-residue polypeptide MB(i + 4)EK. Measurements on MB(i + 4)EK demonstrate that 53% of the initial C' polarization was transferred to the cross peaks at 7.05 T under 25 kHz MAS spinning.
Type
journal article