利用生物技術大量產製瘤胃細菌纖維分解酵素複合體
Date Issued
2002
Date
2002
Author(s)
DOI
902313B002313
Abstract
Attempts were made to examine enzymes
activity after addition cellulose binding
protein (CBP). Cellulase and CBP were
purified from rumen cellulytic bacterium F..
succinogenes S85, and the gene encoding
xylanase C (XynC) of rumen cellulytic
bacterium R. albus 7 was be the source of
xylanase. The gene encoded xylanase was 62
kDa, and detected high xylanase activity.
Cellulase from F.. succinogenes S85, after
passing ion exchange and gel filtration
columns, present two protein peaks with
cellulase activity, and the molecular weight
was approximately 120 and 65 kDa,
respectively. CBP was eluted with sodium
phosphate buffer containing 10% cellobiose
and 1% CHAPS. Enzymes activity were
increasing from four to ten times after
addition of CBP. The result showed that CBP
can effect on both cellulytic and
hemicellulytic enzyme from different bacteria
species and improve their enzyme activity.
Subjects
cellulase
xylanase
cellulose
binding protein
enzyme activity
Publisher
臺北市：國立臺灣大學動物科學技術學系暨研究所
Type
report
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