Formation of N-(2-hydroxyethyl)valine in human hemoglobin - Effect of lifestyle factors
Journal
Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Journal Volume
559
Journal Issue
1月2日
Pages
73-82
Date Issued
2004
Author(s)
Abstract
The formation of N-(2-hydroxyethyl)valine (HEV) in hemoglobin has been considered as a biomarker to assess exogenous and endogenous exposures to ethylene oxide (EO) and/or ethylene (ET). Factors associated with daily exposures to such compounds might significantly affect the formation of HEV. Tobacco smoke containing EO elicited a significant increase in the levels of HEV amongst smokers, although other factors related to lifestyles may warrant further studies. The objective of this study was to specifically analyze HEV using a modified Edman degradation technique in order to study the association between lifestyle related factors (smoking, second-hand smoke exposure, tea and alcohol consumption) and HEV formation in vivo. Total of 148 Taiwanese volunteers with no history of occupational exposure to either EO or ET were recruited in this study. The HEV levels for smokers (204±151pmol HEV/g globin, n=70) were greater than those for non-smokers (57±46pmol HEV/g globin, n=78), HEV level increasing with the number of cigarettes smoked by subjects per day with a rate of 8.8pmol HEV/g globin per cigarettes per day. Further analysis revealed that the rate of HEV formation in our study subjects was significantly associated with the number of daily cigarettes smoked (P<0.001), but was not associated with tea or alcohol consumption, second-hand smoke exposure, subject age, or subject gender. These results suggest that the significantly higher levels of HEV for smokers than for non-smokers were mainly due to subject exposure to EO contained in cigarette smoke. © 2004 Elsevier B.V. All rights reserved.
SDGs
Type
journal article