A Study on the Automated Purification of Biomolecules by Multiterminal Gel Electrophoresis
Date Issued
2008
Date
2008
Author(s)
Kao, Chun-Feng
Abstract
Biomolecule purification is an important step for biochemical experiments and there is an increasing need to develop automated purification system. For this reason, automated purification of biomolecules by multiterminal gel electrophoresis is investigated in this study. PMMA-based, multiterminal electrophoresis device with dimensions of 50 mm x 100 mm x 10 mm was fabricated by machining in this work. The device featured a central channel for electrophoresis separation and two crossing channels for changing electrophoresis direction. The two LED/photodiode sensors were integrated with the device for automatic sample recovery.For demonstration, a mixture of three negatively charged dyes was tested in experiments of separation and purification. It was demonstrated that the dyes could be transported to different terminals sequentially within 30 min. Also, it was found that the dye recovery rate, ranged between 56% and 84%, was dependent on the electrophoresis distance: The longer path the dye traveled, the less the recovery rate was obtained.In addition to the dye molecules, automated separation of DNA fragments were done by a slight modification of the above device format. Accordingly, it is expected that the automated platform based on electrophoresis purification of biomolecules can be applied to on-chip screening and recovery applications.
Subjects
automated separation
biomolecule recovery
cross channels
gel electrophoresis
multiterminal biochip
Type
thesis
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