Development of SSR markers for variety identification of cole crops (Brassica oleracea L.)
Date Issued
2010
Date
2010
Author(s)
Chou, Yi-Cheng
Abstract
Twenty six SSR markers are screened by 65 cultivars of Brassica oleracea L. from seven botanical varieties, with 98 plants tested in total. The objectives of this study are to establish a set of SSR markers to distinguish the majority of cole crops (Brassica oleracea), and to serve as a base for including molecular markers as a tool in practical variety identification in cole crops. The genetic relationships between different varieties are also discussed. The 26 primer pairs amplified 103 alleles in total in all plants, the . number of alleles per locus of the selected makers ranged from 2 to 12, with an average of 3.96. Number of genotypes detected by each marker ranged from 2 to 27, with an average of 6.92.The PIC values of different markers range from 0.2635 to 0.8488, with an average of 0.5399.The 26 SSR markers could distinguish all cultivars in this study, the genetic distances between pair-wise cultivars range from 0.098 to 0.8605, with an average of 0.651. Principal coordinate analysis (PCoA) and UPGMA cluster showed similar results, all the 65 cultivars separated into seven groups as the taxonomy of botanical varieties, i.e. Chinese kale, broccoli, cauliflower, kohlrabi, cabbage, Brussels sprouts, and kale. Two Broccolini cultivars were classified to the broccoli group. According to the results of genetic distance and cluster analysis of different varieties, broccoli and cauliflower have the closest phylogenetic relationship, Chinese kale is the most distant variety from the other, however, still in same species. The result of intra-cultivar variations indicates that pre-knowledge of the genetic variability within a cultivar is needed before the application of molecular markers in actual variety identification in cole crops.
Subjects
Brassica
microsatellite
capillary electrophoresis
Principal Coordinate Analysis
UPGMA
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