斜紋夜蛾細胞培養液之改良

The imprivement of media for
culturing cells from Spodoptera litura.
Cheng-Jen Shih, Department of
Entomology, National Taiwan
University
A cell line derived from Spodoptera
litura, SL7B, was used to ascertain for the
improvement of media for mass production
of Autograph californica
nucleopolyhedrovirus (AcMNPV). Results
revealed that SL7B cell line was better than
commercial cell line, SF21AE, for the
replication of wild type AcMNPV and
recombinant virus protein expressed. SL7B
cells can grow in low cost and simple ISC-03
medium, the speed of cell growth was
increased upon the increase of concentration
of fetal bovine serum (FBS) contained in
cultural medium. In addition, the production
of wild type AcMNPV and amount of
recombinant protein luciferase expressed had
positive correlation to the concentration of
FBS in medium. ISC-03 medium
supplemented with Pluronic F68 was helpful
to the growth of SL7B cells. When Pluronic
F-68 added to the medium containing 2%
FBS, the growth of cell was faster than the
medium containing 8% FBS. Moreover, in
the medium containing same concentration of
FBS, both of wild type AcMNPV and
recombinant protein production were much
higher when Pluronic F-68 was
supplemented.