Visualizing the DNA repair process by a photolyase at atomic resolution
Journal
Science (New York, N.Y.)
Journal Volume
382
Journal Issue
6674
Date Issued
2023-12
Author(s)
Wang, Po-Hsun
Nango, Eriko
Hosokawa, Yuhei
Saft, Martin
Furrer, Antonia
Yang, Cheng-Han
Gusti Ngurah Putu, Eka Putra
Wu, Wen-Jin
Emmerich, Hans-Joachim
Caramello, Nicolas
Franz-Badur, Sophie
Yang, Chao
Engilberge, Sylvain
Wranik, Maximilian
Glover, Hannah Louise
Weinert, Tobias
Wu, Hsiang-Yi
Lee, Cheng-Chung
Huang, Wei-Cheng
Huang, Kai-Fa
Chang, Yao-Kai
Liao, Jiahn-Haur
Weng, Jui-Hung
Gad, Wael
Chang, Chiung-Wen
Pang, Allan H
Yang, Kai-Chun
Lin, Wei-Ting
Chang, Yu-Chen
Gashi, Dardan
Beale, Emma
Ozerov, Dmitry
Nass, Karol
Knopp, Gregor
Johnson, Philip J M
Cirelli, Claudio
Milne, Chris
Bacellar, Camila
Sugahara, Michihiro
Owada, Shigeki
Joti, Yasumasa
Yamashita, Ayumi
Tanaka, Rie
Tanaka, Tomoyuki
Luo, Fangjia
Tono, Kensuke
Zarzycka, Wiktoria
Müller, Pavel
Alahmad, Maisa Alkheder
Bezold, Filipp
Fuchs, Valerie
Gnau, Petra
Kiontke, Stephan
Korf, Lukas
Reithofer, Viktoria
Rosner, Christian Joshua
Seiler, Elisa Marie
Watad, Mohamed
Werel, Laura
Spadaccini, Roberta
Yamamoto, Junpei
Iwata, So
Zhong, Dongping
Standfuss, Jörg
Royant, Antoine
Bessho, Yoshitaka
Essen, Lars-Oliver
Tsai, Ming-Daw
Abstract
Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the structural mechanism of the photolyase-catalyzed repair of a cyclobutane pyrimidine dimer (CPD) lesion using time-resolved serial femtosecond crystallography (TR-SFX). We obtained 18 snapshots that show time-dependent changes in four reaction loci. We used these results to create a movie that depicts the repair of CPD lesions in the picosecond-to-nanosecond range, followed by the recovery of the enzymatic moieties involved in catalysis, completing the formation of the fully reduced enzyme-product complex at 500 nanoseconds. Finally, back-flip intermediates of the thymine bases to reanneal the DNA were captured at 25 to 200 microseconds. Our data cover the complete molecular mechanism of a photolyase and, importantly, its chemistry and enzymatic catalysis at work across a wide timescale and at atomic resolution.
Type
journal article