Evaluation of modification capability of goat milk oligosaccharides on mouse intestinal bacterial composition and immunity
Date Issued
2008
Date
2008
Author(s)
Sun, Peng-Cheng
Abstract
The purpose of this study was to investigate the effect of dietary supplementation of goat milk OS on intestinal bacterial composition and immunity of mice. The goat milk OS were isolated and concentrated by FPLC in NTU Animal Nutrition Lab. Forty male BALB/c mice of 4 weeks age were used in this study. They were randomly allocated to 4 treatments of 10 mice each treatment. The four treatments were: (1)control, (2)galacto-oligosaccharides(GOS), (3)acidic goat milk oligosaccharides(AOS) and (4)neutral goat milk oligosaccharides(NOS). After four weeks of OS feeding, all mice were subjected to the delayed-type hypersensitivity test. After eight weeks, blood samples were collected for assay of the blood cells numbers, immunoglobulin G, immunoglobulin M and splenic lymphocyte proliferation, and samples of the (1)colic content, (2)colic villus, (3)cecal content, (4)cecal villus and (5)feces were taken for culturing count of Bifidobacterium, Lactobacillus, Clostridium perfringens and Escherichia coli. esults showed that all three OS treatments were higher in quantities of leukocytes, lymphocytes, and monocytes than the control group. In delayed-type hypersensitivity test, the swollen skin areas were smaller in the GOS, AOS, and NOS groups. Plasma IgG and IgM concentrations of mice in GOS or AOS group were significantly higher than those of control group. The lymphocyte proliferation competence in GOS and AOS groups was higher for T cell proliferation. In the intestinal bacterial composition, the number of Bifidobacterium spp. and Lactobacillus spp. increased significantly and E. coli decreased in the GOS and AOS groups, but there was not difference between NOS and control groups.n conclusion, goat milk oligosaccharides have ameliorative effect on immunity and intestinal bacterial composition, which was similar to the effects of GOS.
Subjects
mouse
goat milk oligosaccharides
immunity
intestinal bacterial composition
Type
thesis
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