Canine Peripheral Nerve Sheath Tumors: Studies on Pathological and Immunohistochemical Characterization
Date Issued
2005
Date
2005
Author(s)
Tu, Yang-Chang
DOI
zh-TW
Abstract
A total of 36 canine peripheral nerve sheath tumors (PNSTs), 23 malignant PNSTs (MPNSTs), and 13 benign PNSTs (BPNSTs) were diagnosed by histological features and immunohistochemical results. In this study, the affected dogs were 17 (54.8%) females and 14 (41.2%) males. The peak incidence of the PNSTs was between 7 to 14 years of age (26/30; 86.7%). Breed at the highest risk was mongrel dogs (17/33; 51.5%). The limbs were the most common site (25/33; 75.6%), followed by the trunk (5/33; 15.2%), genital (2/33; 6.1%), and head (1/33; 3.0%). Microscopically, PNSTs were composed of spindle or S shape cells arranged in wavy bundles, palisading, storiform, and whorl fashions. The predominant tumor cells of MPNSTs were either plump spindle or round in shape with epithelioid characteristics, mixed with multinucleated giant cells occasionally. There were two MPNSTs with cartilaginous metaplasia, and six MPNSTs presented with extensive necrotic foci accompanied with thrombosis in 3 of 6 cases. Regarding to 13 BPNSTs, all exhibited a typical feature of either schwannoma or neurofibroma. Only 2 of 9 schwannomas had typical Antoni type A and B features. The 4 neurofibromas presented with extensive collagen deposition likened to shredded carrots appearance, which tumor cells arranged in hypocellularity. Ultrastructurally, the tumor cells were long, thin, intertwining processes, a duplicated external basal lamina covering the cells, and long spacing collagen. Immunohistochemically, the expression of vimentin (100%), laminin (93.3%), S-100 (87.8%), nerve growth factor receptor (NGFR, 84.8%), neurofilament (51.58%), neuron-specific enolase (NSE, 30.3%), α-SMA (α-smooth muscle actin, 6.1%), and desmin (3.0%) were found in PNSTs. PNSTs failed to demonstrate expression of cytokeratin, factorⅧ, and glial fibrillary acidic protein (GFAP). On conclusion, PNSTs often occur in the skin soft tissue of dogs that share histological similarities with other spindle cell tumors. Comprehensive studies on microscopical findings and immunohistochemical characterization provide a useful and practical method to differentiate PNSTs from other spindle cell tumors.
Subjects
犬
周邊神經鞘膜瘤
免疫組織化學染色
Dog
Immunohistochemistry
Peripheral Nerve Sheath Tumors
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-94-R92629002-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):1f232cf69223674b9e3ec072eb7e2ae9