The study of the effects of mechanical compression articular cartilage tissue engineering.
Date Issued
2005
Date
2005
Author(s)
Chou, Hsang-Hong
DOI
zh-TW
Abstract
The goal of this study was to evaluate the effect of dynamic mechanical compression on articular cartilage tissue engineering. We designed a compressive bioreactor and fabricated elastic polyurethane (Pellethane) scaffolds with porosity of 82% and pore size ranged from 200 to 350 µm. Chondrocytes mixed with/without type I collagen hydrogels were seeded into scaffolds. After cultured for 5 days, chondrocyte-scaffold complex was exposed to the uniaxial dynamic compression of 0.1 Hz frequency. The compression of 20% and 30% strain was applied for 0, 4, 8, 12, 24 hours. The other protocol was to culture chondrocyte-scaffold complex for periods of 0, 2. 6, 12, 24 hours respectively after exposed to a 30% strain compression for 12 hours. The cellular response was assessed using RT-PCR assay to quantify expression of type I collagen, type II collagen and aggrecan. We found that the relative intensity of aggrecan was increased with loading duration and maintained for a while after compression, while there was no significant effect on type I and type II collagen gene expression. This system of dynamic compression and elastic polyurethane scaffold may be contributed to the application of engineered cartilage.
Subjects
關節軟骨
組織工程
機械性壓縮
第I型膠原蛋白
第II型膠原蛋白
aggrecan
articular cartilage
tissue engineering
mechanical compression
type I collagen
type II collagen
Type
thesis
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