Metal interference on luciferase activity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in bioassays of recombinant mouse hepatoma cells
Resource
Journal of Hazardous Materials 165 (1-3): 881-885
Journal
Journal of Hazardous Materials
Journal Volume
165
Journal Issue
1-3
Pages
881-885
Date Issued
2009
Author(s)
Abstract
This study will examine the effect of a series of metals on the luciferase activity expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in H1L6.1 cells. A 2-level factorial design was employed to identify major influential metal species of four test metals (As5+, Cr6+, Cd2+, and Pb2+) in the chemically activated luciferase expression (CALUX) of H1L6.1 cells. The observed data revealed that Cr6+ exerted a significant inhibiting effect on the luciferase activity. The impact of key metal species at various concentrations was analyzed for determination of the maximum tolerable level for H1L6.1 cell. It was observed that Cr6+ lowered the luciferase activity by 36.1 and 65.4%, at levels of 1.0 and 1.5 μg-Cr/mL-medium (p < 0.05), respectively, but showed no significant difference at higher Cr6+concentrations (2.0-5.0 μg-Cr/mL-medium). ? 2008 Elsevier B.V. All rights reserved.
Subjects
Bioassays; CALUX; Metals; PCDDs; PCDFs
SDGs
Other Subjects
CALUX; Factorial designs; Hepatoma cells; Inhibiting effects; Luciferase activities; Luciferase expressions; Metal species; Observed datum; PCDDs; PCDFs; Tetrachlorodibenzo p dioxins; Chromium; Lead; Metals; Enzyme activity; 2,3,7,8 tetrachlorodibenzo para dioxin; arsenic acid; cadmium; chromium; lead; luciferase; metal; bioassay; cell organelle; concentration (composition); enzyme activity; gene expression; PCDD; PCDF; rodent; animal cell; article; bioassay; cancer survival; controlled study; enzyme activity; factorial design; hepatoma cell; nonhuman; protein expression; Animals; Arsenic; Cadmium; Carcinoma, Hepatocellular; Cell Line, Tumor; Chromium; Dose-Response Relationship, Drug; Gene Expression Regulation; Lead; Luciferases; Metals; Mice; Tetrachlorodibenzodioxin; Transfection
Type
journal article
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