Studies on Isolation, Identification, and Production of Antimicrobial Compound Heyneanol A from Taiwan Wild Grape (Vitis thunbergii var. taiwaniana)
Date Issued
2008
Date
2008
Author(s)
Peng, Szu-Chin
Abstract
The aim of this study was to determine the antimicrobial compounds against pathogenic bacteria from grapevines and to product these active compounds from Vitis plants. We first analyzed the antimicrobial activity of leaf, vine, and root extracts from five wild grape species such as taiwan wild grape (Vitis thunbergii Sieb. et Zucc.), hancei wild grape (Ampelopsis brevipedunculata var. hancei), muscadine grape (V. rotundifolia var. jumbo), and kelung wild grape (V. kelungensis) using the disc diffusion method. The result showed that root extracts of Vitis species all inhibited Gram-positive bacteria such as Bacillus subtilis, Staphylococcus aureus and methicillin-resistant S. aureus (MRSA). The root extracts of taiwan wild grape (Vitis thunbergii var. taiwaniana), which showed markedly antimicrobial activities (minimum inhibitory concentration, MIC value of 64 μg/mL toward S. aureus), and contained three possible active compounds were further collected using preparative thin layer chromatography with recrystallization methods. One of these three purified compounds was confirmed as Heyneanol A using ultraviolet, mass spectroscopy and nuclear magnetic resonance analyses. Heyneanol A showed an MIC value of 2 μg/mL toward MRSA, S. aureus, Enterococcus faecium, and B. subtilis and that of 4 to 8 μg/mL for S. epidermidis, Streptococcus agalactiae, Str. pyogenes, and Str. equines. Moreover, the contents of Heyneanol A in root extracts were determined as 37 mg/g dry weight (DW) of taiwan wild grape, 25 mg/g DW of kelung wild grape, 16 mg/g DW of kyoho grape (V. vinifera X V. labrusca cv. Kyoho), 14 mg/g DW of 5C grape (V. berlandieri X V. riparia cv. 5C), and 5 mg/g DW of muscadine grape using high-performance liquid chromatography. Furthermore, to product roots of grapevines in a convenient way, we used the trough system to cultivate taiwan wild grape and analyzed the effects of root training on the growth, distribution, and Heyneanol A content of roots of taiwan wild grape. After three years of training, the roots of treated grapevines were significantly longer than those roots of untreated vines and the distribution of treated roots was more concentrated, which made the harvest work much easier and time-saving. In addition, the content of Heyneanol A within the roots of treated vines was 39.9 mg/g DW, and showed no significant difference with 36.5 mg/g DW of the content of untreated vines. These results suggest that the trough system could be used for root production of taiwan wild grape.
Subjects
Vitis thunbergii
Vitis rotundifolia
Vitis kelungensis
antimicrobial activity
methicillin-resistant Staphylococcus aureus (MRSA)
grape root
trough system
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-97-F91628102-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):2f4eac291528b2dded668f8983aebd24