Hsp27 decreases inclusion body formation from mutated GTP-cyclohydrolase I protein
Journal
Biochimica et Biophysica Acta - Molecular Basis of Disease
Journal Volume
1782
Journal Issue
3
Pages
169-179
Date Issued
2008
Author(s)
Abstract
GTP cyclohydrolase I (GCH), an oligomeric protein composed of 10 identical subunits, is required for the synthesis of neurotransmitters; mutations in GCH are associated with dopa-responsive dystonia (DRD) and hyperphenylalaninemia. Mutated GCH proteins are unstable and prone to dominant-negative effect. We show herein that expression of the GCH mutant GCH-201E or the splicing variant GCH-II caused intracellular inclusion bodies. When Hsp27 was expressed together with the GCH mutants, Hsp27 expression decreased the formation of inclusion bodies by GCH (as assessed by immunofluorescence) and decreased the amount of insoluble GCH mutant proteins (as assessed by Western blot). Transfection of pcDNA-Hsp27-S3D, a phosphorylation-mimicry Hsp27 mutant, was more effective at the mutated GCH proteins than transfection with pcDNA-Hsp27, but okadaic acid, a phosphatase inhibitor, enhanced the effect of pcDNA-Hsp27. Hsp27-S3D also abolished the dominant-negative action of GCH-II. The mutated GCH proteins interacted with the wild-type GCH protein; the inclusion bodies were positive for lysosomal marker LAMP1, soluble in 2% SDS, and were not ubiquitinated. Phophorlyated Hsp27 also decreased the inclusion body formation by the huntingtin polyglutamines. Therefore, diseases involving mutated oligomeric proteins would be manageable by chaperone therapies. ? 2008 Elsevier B.V. All rights reserved.
Subjects
Chaperone; Dopa-responsive dystonia; GTP-cyclohydrolase I; Heat shock protein 27; Inclusion body formation; Phosphorylation
SDGs
Other Subjects
guanosine triphosphate cyclohydrolase I; heat shock protein 27; okadaic acid; animal cell; article; cell inclusion; controlled study; immunofluorescence; nonhuman; priority journal; protein expression; protein phosphorylation; Western blotting; wild type; Animals; Cells, Cultured; Cricetinae; Fluorescent Antibody Technique; GTP Cyclohydrolase; Heat-Shock Proteins; Inclusion Bodies; Mutation; Neoplasm Proteins; Okadaic Acid; Phosphorylation; Transfection
Type
journal article