Wild bitter gourd extract up-regulates mRNA expression of PPAR alpha, PPAR gamma and their target genes in C57BL/6J mice
Resource
JOURNAL OF ETHNOPHARMACOLOGY, 135(1), 156-161
Journal
JOURNAL OF ETHNOPHARMACOLOGY
Journal Volume
135
Journal Issue
1
Pages
156-161
Date Issued
2011
Date
2011
Author(s)
Abstract
Ethnopharmacological relevance: Wild bitter gourd (Momordica charantia Linn. var. abbreviata ser.) was commonly used as a medicinal herb in Asia, Africa, and South America because of its anti-diabetic, antibacterial, anti-viral, and chemopreventive functions. Materials and methods: C57BL/6J mice were orally administered with 250, 500 or 1000 mg/kg BW of WBGE in 0.2 mL/mouse of olive oil daily for 2 weeks. Results: Compared to control (vehicle treated) mice, mice receiving WBGE showed significantly higher PPARα, ACO (acyl-CoA oxidase) and L-FABP (liver-fatty acid binding protein) mRNA expression, ACO activity and protein in the liver (P < 0.05), as clofibrate-treated mice. WBGE treatment also resulted in significantly higher PPARγ and LPL (lipoprotein lipase) mRNA (P < 0.05) in the epididymal adipose tissue. Liver triglyceride and non-esterified fatty acid concentration in WBGE treated mice were significantly lower than those of control mice (P < 0.05). Plasma adiponectin level was significantly higher in mice receiving WBGE than in control mice (P < 0.05), as the rosiglitazone treated mice. Conclusion: Results of this study demonstrated that WBGE also activates PPARα and PPARγ signaling pathway in vivo. ? 2011 Elsevier Ireland Ltd. All rights reserved.
Subjects
Adiponectin; PPARα; PPARγ; Wild bitter gourd
SDGs
Other Subjects
acyl coenzyme A oxidase; adiponectin; antidiabetic agent; binding protein; clofibrate; fatty acid; lipoprotein lipase; liver enzyme; liver fatty acid binding protein; messenger RNA; Momordica charantia extract; olive oil; peroxisome proliferator activated receptor alpha; peroxisome proliferator activated receptor gamma; rosiglitazone; triacylglycerol; unclassified drug; animal experiment; animal tissue; article; blood sampling; body weight; C57BL 6 mouse; concentration (parameters); controlled study; diet supplementation; epididymis fat; gene expression; gene targeting; in vivo study; male; metabolic disorder; Momordica charantia; mouse; nonhuman; reverse transcription polymerase chain reaction; signal transduction; upregulation; Western blotting; Acyl-CoA Oxidase; Adiponectin; Adipose Tissue; Animals; Clofibrate; Epididymis; Fatty Acid-Binding Proteins; Fatty Acids, Nonesterified; Fruit; Gene Expression; Hypoglycemic Agents; Hypolipidemic Agents; Lipoprotein Lipase; Liver; Male; Mice; Mice, Inbred C57BL; Momordica charantia; Plant Extracts; PPAR alpha; PPAR gamma; RNA, Messenger; Thiazolidinediones; Triglycerides; Up-Regulation; Abbreviata; Momordica charantia; Mus
Type
journal article
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