Effects of C/N ratios on Growth, Water Quality and Mutual Inhibition of Probiotic and Vibrio parahaemolyticus
Date Issued
2015
Date
2015
Author(s)
Kao, Tai-Yun
Abstract
This study added molasses as carbon source into the water to control carbon/ nitrogen ratios at five levels, 0, 5, 10, 15 and 20. 0 added nothing as a control group. To investigate different carbon/ nitrogen ratios effects of Bacillus subtilis, Rhodobacter sphaeroides and Vibrio parahaemolyticus on growth, water quality and mutual inhibition. In the experiment of the bacteria growth in different carbon/ nitrogen ratios water, the results show that Bacillus subtilis at 20 group were significantly higher than the control (p < 0.05). Each treatment group increased growth of Rhodobacter sphaeroides significantly compared to the control group (p < 0.05), while 15 treatment group had better effect than all treatment group. In addition, Vibrio parahaemolyticus at 5 treatment group were significantly increased rate of growth to control (p < 0.05). In the experiment of the water quality of bacteria in different carbon/ nitrogen ratios water, the results indicated that Bacillus subtilis reduced ammonia-N concentrations in water, but it was not effects significantly on each treatment group. In addition, the nitrite-N concentrations significantly lower at 20 treatment group than control (p < 0.05). Each treatment group of Rhodobacter sphaeroides, which reduced ammonia-N and nitrite-N concentration significantly compared to the control group (p < 0.05), while 10、15 and 20 treatment had better results. Vibrio parahaemolyticus at 15、20 treatment reduce the ammonia-N and nitrite-N concentrations significantly than control group (p < 0.05). Through the inhibition of bacteria growth, 103、105 cfu/ ml of Bacillus subtilis can produce inhibition effects on 103 cfu/ ml Vibrio parahaemolyticus. The 105 cfu/ ml of Rhodobacter sphaeroides had inhibition effects on 103 cfu/ ml Vibrio parahaemolyticus after 72 hr. In conclusion of this study, carbon source addition increase carbon/ nitrogen ratios in water can effectively improve the water quality and growth activities of Bacillus subtilis and Rhodobacter sphaeroides. To build a suitable environment of probiotic for more efficiently inhibit the growth of Vibrio parahaemolyticus.
Subjects
Carbon/ Nitrogen Ratios
Bacillus subtilis
Rhodobacter sphaeroides
Vibrio parahaemolyticus
Growth
Water quality
Inhibition
Type
thesis
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