Studies on chemical constituents and anti-inflammatory activity of cultivated Armillariella mellea
Date Issued
2011
Date
2011
Author(s)
Chang, Ching-Wen
Abstract
Armillariella mellea was reported to exhibit anti-inflammatory activity, especially that of the polysaccharide (PS) . To mass produce high quality of A. mellea was the aim of the study. The maximum production of 15.73 ± 0.16 g/l was obtained at 49 days, with the yield of PS and ethanolic extract of 0.63 ± 0.04 g/l and 5.51 ± 0.38 g/l, respectively. Galactose and glucose was the major sugar of PS with value of 1071.30 ± 10.89 and 525.45 ± 4.14 mmol/g PS at 49-day-culture, respectively. The components of ethanolic extract included ADP, cytidine and adenosine with the value of 0.48, 0.34 and 0.74 mg/mg ethanolic extract, respectively. Effects of extracted PS, sulfated polysaccharide (SPS) and ethanolic extract from cultured A. mellea on lipopolysaccharide (LPS) and TNF-a induced inflammation-related cytokines in RAW264.7 macrophages and EAhy926 were evaluated. Secretions of tumor necrosis factor-a (TNF-a) and interleukin-6 (IL-6) were detected in RAW264.7. The results showed that inhibition of TNF-a and IL-6 production were in a dose-dependent manner with ethanolic extract of A. mellea. PS from 49-day-cultured mycelia at 500 mg/ml showed significant inhibition of TNF-a and IL-6 in the value of 31.17% and 62.74%, respectively. Furthermore, SPS exhibited more effective on inhibition of TNF-a and IL-6 secretion as compared that with non-SPS in the value of 100% and 56.82% suppression, respectively. On the other hand, the secretion of cytokine monocyte chemotactic protein-1 (MCP-1) was significantly reduced in 52.17%, 37.49% and 34.53% for 49-day-culture PS, ethanolic extract and sulfated-PS (SPS), respectively. All of the above results suggested that A. mellea exhibited anti-inflammatory activity. In conclusion, in our cultured condition, A. mellea''s dry weight and PS production could be enhanced at 49-cultured days. Glactose and glucose was the major component of A. mellea PS. PS, ethanolic extract and SPS of A. mellea significantly inhibited the release of the inflammatory related cytokines of TNF-a, IL-6 and MCP-1.
Subjects
Armillariella mellea
anti-inflammatory
TNF-a
IL-6
MCP-1
IkB-a
Type
thesis
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