Slide-free histopathological imaging of hematoxylin-eosin-stained whole mount tissues using Cr:forsterite laser-based nonlinear microscopy
Journal
Proceedings of SPIE - The International Society for Optical Engineering
Journal Volume
11026
Date Issued
2019
Author(s)
Abstract
Intraoperative margin assessment during Mohs micrographic surgery (MMS) is clinically critical. Currently, frozen pathology is the gold standard of assessing Mohs excisions for signs of remaining cancerous lesions; however, it is still considered time-consuming and sometimes misses the real margins. In this study, we demonstrate nonlinear microscopy imaging of hematoxylin-eosin (H&E) stained whole-mount skin tissues with a sub-femtoliter resolution through third-harmonic-generation (THG) and three-photon-excited-fluorescence (3PF) by using 1260 nm Cr:forsterite-laser-based nonlinear microscopy, which also enables virtual biopsy for preoperative margin assessment. Without the physical sectioning procedure and with a simplified staining process, real margins were preserved and time before microscopic examination was potentially saved three times shorter than frozen pathology. By exactly stained by H&E, the compatibility allows the adjunction to frozen pathology if further examination is needed. Virtual-sectioning imaging of H&E stained skin tissues is displayed real-time and can be post-processed analogously to conventional H&E histology, facilitating pathologists for diagnosis. ? 2019 SPIE. Downloading of the abstract is permitted for personal use only.
Subjects
Cr:forsterite; Eosin; Hematoxylin; Histopathology; Mohs surgery; Nonlinear microscopy; Third harmonic generation; Three-photon fluorescence
SDGs
Other Subjects
Chromium compounds; Fluorescence; Histology; Multiphoton processes; Nonlinear optics; Olivine; Pathology; Photons; Surgery; Tissue; Cr: forsterite; Eosin; Hematoxylin; Histopathology; Mohs surgery; Nonlinear microscopy; Three-photon; Harmonic generation
Publisher
SPIE
Type
conference paper