Drosophila Decapping Protein 1, dDcp1, Is a Component of the oskar mRNP Complex and Directs Its Posterior Localization in the Oocyte
Resource
Developmental Cell 10 (5): 601-613
Journal
Developmental Cell
Journal Volume
10
Journal Issue
5
Pages
601-613
Date Issued
2006
Date
2006
Author(s)
Abstract
In Drosophila, posterior deposition of oskar (osk) mRNA in oocytes is critical for both pole cell and abdomen formation. Exon junction complex components, translational regulation factors, and other proteins form an RNP complex that is essential for directing osk mRNA to the posterior of the oocyte. Until now, it has not been clear whether the mRNA degradation machinery is involved in regulating osk mRNA deposition. Here we show that Drosophila decapping protein 1, dDcp1, is a posterior group gene required for the transport of osk mRNA. In oocytes, dDcp1 is localized posteriorly in an osk mRNA position- and dosage-dependent manner. In nurse cells, dDcp1 colocalizes with dDcp2 and Me31B in discrete foci that may be related to processing bodies (P bodies), which are sites of active mRNA degradation. Thus, as well as being a general factor required for mRNA decay, dDcp1 is an essential component of the osk mRNP localization complex. © 2006 Elsevier Inc. All rights reserved.
Subjects
DEVBIO; RNA
Other Subjects
Drosophila Decapping protein 1; Drosophila protein; messenger RNA; oskar protein; ribonucleoprotein; unclassified drug; article; cellular distribution; controlled study; Drosophila; embryo; exon; genetic code; nonhuman; oocyte development; priority journal; protein content; protein degradation; protein determination; protein function; protein localization; protein processing; regulatory mechanism; RNA degradation; RNA transport; Amino Acid Sequence; Animals; Cytoplasm; DNA-Binding Proteins; Drosophila melanogaster; Drosophila Proteins; Egg Proteins; Genes, Insect; Microtubule-Associated Proteins; Microtubules; Molecular Sequence Data; Oocytes; Protein Transport; Ribonucleoproteins; RNA Stability; RNA, Messenger; RNA-Binding Proteins; Sequence Alignment; Transcription Factors
Type
journal article
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