Immunomodulatory and Antioxidative Activity of Lactic Acid Fermented Defatted Sesame Meal

Sesame meal obtained from oil extraction of sesame. The main composition is protein and was commonly used as feed ingredients or compost for domestic animals. Sesame meal contains lignan compounds which have been proven to exert many biological activties. Studies indicated that sesaminol triglucoside in sesame seeds are the major lignans that display biological activities. The antioxidative activities of sesaminol triglucoside were weak in vitro, but they have been reported to suppress oxidative stress after being metabolized or hydrolized in vivo. Lactic acid bacteria (LAB) have high β-glucosidase activity which can hydrolyze the glycosides attached to the lignans. Moreover, LAB also exhibit several bio-functions. In this study, the sesame meal were first prepared by cultured with lactic acid bacteria such as Lactobacillus plantarum BCRC 11697, Lactobacillus delbrueckii subsp. bulgaricus BCRC 10696, Lactobacillus acidophilus BCRC 14079, Lactobacillus rhamnosus GG ATCC 53103, Streptococcus thermophilus BCRC 14087, Lactobacillus casei 01 and Lactococcus lactis, which to enhance the function of sesame meal. The growths of various LAB in sesame meal were observed. The antioxidative and immunomodulatory activity of water extract and ethanol extract of lactic acid bacteria fermented defatted sesame meal were further determined.esults showed that L.delbrueckii subsp. bulgaricus BCRC 10696 and L. casei 01 have higher cell counts, lower pH, higher titratable acidity, and higher deglycosilation activity. Generally, ethanol extract exhibited stronger antioxidant actvity than water extracts. Additionally,the ethanol extract of L. delbrueckii subsp. bulgaricus BCRC 10696 showed the strongest antioxidative of EC50 for DPPH(160.6 ± 6.50 μg /mL), TEAC(0.34± 0.02 mM Trolox equiv.), ORAC(1.06 ± 0.02 mM Trolox equiv.), and also contains the highest concentration of total polyphenols.ater extracts showed the highest immune promotion activity in immunoregulation assays. S. thermophilus BCRC 14087 exhibited the highest activity which increased the secretion of TNF-α. However, 95% ethanol extracts of L. delbrueckii subsp. bulgaricus BCRC 10696 exerted strongest anti-inflammation activity, with , of which under 100 μg/mL, TNF-α was decreased by 31.25 %, and IL-6 decreased by 36.5 %.o further confirm the results, a sesame meal extract was used. Results indicated that the increased of antioxidative activity in fermented sesame meal were due to deglycosylated of sesaminol triglucoside. In addition, the results illustrated that small molecules are responsible for the immunoregulatory effect other than the Maillard reaction products or sesame lignans.