CTPS forms the cytoophidium in zebrafish.
Journal
Experimental cell research
Journal Volume
405
Journal Issue
2
ISSN
1090-2422
Date Issued
2021-08-15
Author(s)
Keppeke, Gerson Dierley
Antos, Christopher L
Peng, Min
Andrade, Luis Eduardo Coelho
Liu, Ji-Long
Abstract
Cytidine triphosphate synthase (CTPS) catalyzes the rate-limiting step of de novo CTP biosynthesis. An intracellular structure of CTPS, the cytoophidium, has been found in many organisms including prokaryotes and eukaryotes. Formation of the cytoophidium has been suggested to regulate the activity and stability of CTPS and may participate in certain physiological events. Herein, we demonstrate that both CTPS1a and CTPS1b in zebrafish are able to form the cytoophidium in cultured cells. A point mutation, H355A, abrogates cytoophidium assembly of zebrafish CTPS1a and CTPS1b. In addition, we show the presence of CTPS cytoophidia in multiple tissues of larval and adult fish under normal conditions, while treatment with a CTPS inhibitor 6-diazo-5-oxo-l-norleucine (DON) can induce more cytoophidia in some tissues. Our findings reveal that forming the CTPS cytoophidium is a natural phenomenon of zebrafish and provide valuable information for future research on the physiological importance of this intracellular structure in vertebrates.
Subjects
CTPS
Cytoophidium
Zebrafish
Publisher
Elsevier
Description
Article number: 112684
Type
journal article