Systematic synthesis and identification of monolignol pathway metabolites
Journal
New Phytologist
Journal Issue
Online Version of Record before inclusion in an issue
ISSN
0028-646X
1469-8137
Date Issued
2024-09-12
Author(s)
Chung‐Ting Kao
Fan‐Wei Yang
Meng-Chen Wu
Tzu‐Huan Hung
Chen‐Wei Hu
Chiu‐Hua Chen
Pin‐Chien Liou
Chia‐Chih Chang
Tung‐Yi Lin
Ying‐Lan Chen
Jung‐Chen Su
DOI
10.1111/nph.20101
Abstract
Monolignol serves as the building blocks to constitute lignin, the second abundant polymer on Earth. Despite two decades of diligent efforts, complete identification of all metabolites in the currently proposed monolignol biosynthesis pathway has proven elusive. This limitation also hampers their potential application. One of the primary obstacles is the challenge of assembling a collection of all molecules, because many are commercially unavailable or prohibitively costly. In this study, we established systematic pipelines to synthesize all 24 molecules through the conversions between functional groups on a core structure followed by the application to other core structures. We successfully identified all of them in Populus trichocarpa and Eucalyptus grandis, two representative species respectively from malpighiales and myrtales in angiosperms. Knowledge about monolignol metabolite chemosynthesis and identification will form the foundation for future studies.
Subjects
chemical synthesis
LC-MS/MS
lignin
monolignol biosynthesis
woody angiosperms
Publisher
Wiley
Type
journal article