TGF-ALFA 因子對豬早期胚體外發育及胚內源性基因表現之影響
Date Issued
1998
Date
1998
Author(s)
鄭登貴
DOI
872313B002075
Abstract
In the present study, timely expression of
gene(s) in porcine oviduct epithelia was
identified and the consequent effect of the
specified ligant on expression of embryonic
gene at early stages of development was
elucidated. Total RNA extracted from the
oviduct epithelia of gilts, fallotomized at 50-60,
96 and 120 h post the injection of human
chorionic gonadotrophin (hCG), were subjected
to analyses by mRNA differential display and
by reverse transcription-polymerase chain
reaction (DDRT-PCR), respectively. Results
from sequencing analysis confirmed that the
sequence of gene fragment of (96/120)-1-R4A3,
expressed by porcine oviduct epithelia at 96 and
120 h post the hCG injection, was identical to
that of gene encoding the porcine transforming
growth factor-α(TGF-α). Moreover, by using
an in vitro culture system, it was found that the
expression of TIG-I gene in early 4-cell of
porcine embryos does appear to be TGF-α
dependent. The cDNA sequence of TIG-I gene
was further confirmed to be homologous to that
of mo25 cDNA found in mouse early embryos
and the sequence also appeared to be having a
Ca-binding domain located within the coding
region. These results imply that TGF-α gene
timely expressed in the epithelia of porcine
oviducts is essentially required for porcine early
embryos to activate their signal transduction
pathway.
Publisher
臺北市：國立臺灣大學動物科學技術學系暨研究所
Type
report
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