Quantitative Analysis of Collagen by Capillary Electrophoresis (II)
Date Issued
1998-07-31
Date
1998-07-31
Author(s)
黃玲惠
DOI
872314B002311M08
Abstract
Collagen is the major component of
extracellular matrix, and is also the major
protein of connective tissues. Many diseases
are associated with the changes of collagen
typing and their contents. To establish a more
rapid, sensitive, and accurate method,
capillary electrophoresis was chosen which
combines the advantages of electrophoresis
and high performance liquid chromatography.
It is difficult to differentiate various types of
collagen using previously established method
due to similarity on the amino acid
composition. Variation on certain peptide for
different types of collagen exists after CNBr
digestion treatment. An optimal analytical
condition for CNBr peptides of collagen was
established in the current study.
extracellular matrix, and is also the major
protein of connective tissues. Many diseases
are associated with the changes of collagen
typing and their contents. To establish a more
rapid, sensitive, and accurate method,
capillary electrophoresis was chosen which
combines the advantages of electrophoresis
and high performance liquid chromatography.
It is difficult to differentiate various types of
collagen using previously established method
due to similarity on the amino acid
composition. Variation on certain peptide for
different types of collagen exists after CNBr
digestion treatment. An optimal analytical
condition for CNBr peptides of collagen was
established in the current study.
Subjects
capillary electrophoresis
collagen
CNBr peptides
Publisher
臺北市:國立臺灣大學醫學工程學研究所
Type
report
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