缺氧前置 訓練的機制-用蛋白質學方法探討
Date Issued
2004-07-31
Date
2004-07-31
Author(s)
賴逸儒
DOI
922314B002272
Abstract
Preconditioning is defined as a phenomenon in which an organ or tissue is
rendered resistant to a more sustained insult by prior exposure to brief periods of injury.
Altitude training, used by endurance athletes to improve sea-level performance, is an
exercise under exposure of chronic hypoxia. Exposure of the rat to a period of hypoxia
increases the cardiac tolerance to subsequent ischemic insults. The mechanism of hypoxic
preconditioning remains to be elucidated. We use the proteomics approach to study
thoroughly the important factors involved in the mechanism of hypoxic preconditioning,
Wistar rats exposed to 2 weeks of hypoxia were used, the liver tissue of experiment
animals are harvested as target organ analysis.
Two-dimensional gel electrophoresis was used to pick out the differentially-displayed
protein spots for further analysis by mass spectrometry, and Protein database searching
was performed. The proteins identified included myosin heavy chain, myosin polypeptide
9 (non-muscle type), myosin polypeptide 3, actin alpha1 and cytoplasmic beta-actin
Western blot analysis confirmed the increased expression of myosin heavy chain
(gi|8250661 ) in liver tissue of rats preconditioned by hypoxia.
Subjects
Hypoxia
Liver
myosin
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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