Harmonic generation microscopy of bone microenvironment in vivo
Journal
Optics Communications
Journal Volume
422
Pages
52
Date Issued
2018
Author(s)
Wu, PC
Shen, YF
Liu, TM
CHI-KUANG SUN
Lin, CP
Abstract
Here we report and review the investigation of bone microenvironment in vivo with harmonic generation microscopy. Excited by an infrared femtosecond laser, the second harmonic generation images can reveal the bone structures and boundaries. The third harmonic generation images can reveal the osteocytes, connecting canaliculi, and granular bone marrow cells. These imaging features can be used to observe and analyze the histology of the bone microenvironment in the future.
Subjects
Osteocyte; Bone marrow; Cr:forsterite; Femtosecond; Third harmonic generation
SDGs
Publisher
ELSEVIER SCIENCE BV
Type
journal article