阿拉伯芥中FIN219基因的功能性研究(1/3)
Date Issued
2004-07-31
Date
2004-07-31
Author(s)
DOI
922311B002027
Abstract
FIN219 has been shown to be involved in phytochrome A-mediated signaling pathway
and acts as a suppressor of COP1 in Arabidopsis. To further understand the function of
FIN219 in light signaling during Arabidopsis development, a yeast two-hybrid
approach was utilized to isolate FIN219-interacting partners. A gene FIP1
(FIN219-interacting protein1) encoding a GST(glutathione S-transferase) was
recovered and demonstrated to interact with the C-terminus of FIN219. The transgenic
plants overexpressing the FIP1 gene in wild type Columbia display a hyposensitive
phenotype with longer hypocotyls under continuous far-red and red light and this longer
hypocotyl phenotype is dependent on the presence of the functional FIN219; however,
FIP1 antisense lines show an opposite phenotype, suggesting that FIP1 may act as a
negative regulator for FR-mediated inhibition of hypocotyl elongation. Furthermore,
the results of Northern blot analyses indicated that FIP1 gene expression is upregulated
by white light and detectable in FR as well as B light, but its expression is hardly
detected in both dark and red light condition. Besides, its expression is reduced in
phytochrome A mutants and several light signaling transducer such as fin219, fhy3,
cop1 and hy5 mutants in continuous far red light condition and also decreased in phyB
and fhy1 mutants in continuous R light. Furthermore, FIP1 expression is also induced
by auxin, jasmonate and herbicide safeners. The GUS-FIP1 fusion protein is localized
in both nucleus and cytoplasm based on the result of transient assays in onion cells.
Taking all current results together, FIP1 is interacting with FIN219 and acts as a
crosstalk between light signaling and phytohormone signaling.
Subjects
Phytochrome A
Jasmonate
Publisher
臺北市：國立臺灣大學植物科學研究所
Type
journal article
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