Multiplex polymerase chain reaction assay for the detection of minute virus of mice and mouse parvovirus infections in laboratory mice
Journal
Laboratory Animals
Journal Volume
47
Journal Issue
2
Pages
116-121
Date Issued
2013
Author(s)
Wang, K.W.
Chueh, L.L.
Wang, M.H.
Huang, Y.T.
Fang, B.H.
Chang, C.Y.
Fang, M.C.
Chou, J.Y.
Abstract
Mouse parvoviruses are among the most prevalent infectious pathogens in contemporary mouse colonies. To improve the efficiency of routine screening for mouse parvovirus infections, a multiplex polymerase chain reaction (PCR) assay targeting the VP gene was developed. The assay detected minute virus of mice (MVM), mouse parvovirus (MPV) and a mouse housekeeping gene (α-actin) and was able to specifically detect MVM and MPV at levels as low as 50 copies. Co-infection with the two viruses with up to 200-fold differences in viral concentrations can easily be detected. The multiplex PCR assay developed here could be a useful tool for monitoring mouse health and the viral contamination of biological materials.
Type
journal article